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Quick Details

  • CAS No.: 9014-01-1
  • Other Names: Proteinase, high-end alkaline proteinase, bacillus licheniformis, hig
  • MF: NULL
  • EINECS No.: 3.4.21.62
  • Place of Origin: Shandong, China (Mainland)
  • Type: Feed Grade Antibiotic & Antibacterial Agents, Feed Grade Enzymes, TTX Keratinase as a high quality alkaline protease
  • Efficacy: Promote Healthy & Growth, Promote Nutrition, TTX Keratinase as a high quality alkaline protease
  • Brand Name: TTX
  • Model Number: Powder
  • Activity:: 200,000 U/g
  • Form:: Powder
  • Color:: Kelly
  • Particle size:: More than 80% pass through standard sieve of 40 meshes
  • Optimum pH:: 11
  • pH range:: Relative activity remains on above 60% at pH 7-12.5
  • Optimum temperature:: 60 degree centigrade
  • Temperature range:: Relative activity remains on above 60% at T 45-65℃
  • Alkali resistance:: Activity is above 80% after alkali treatment for 4 hours at pH 6-12
  • Heat resistance:: Activity is above 80% after heat-treatment for 10 min at T20-65℃.

Packaging & Delivery

Packaging Details: Packaging: 25kg/bag, 20kg/bag, 25kg/drum, 20kg/drum or as requirement
Port of loading: Qingdao, China by sea or as requirement
One 20'GP can contain 10 -13 tons if bag for keratinase
Delivery Detail: 10-20 days after confirmed contract

China factory supplier keratinase enzyme feed additive 

Product Description

 Description summary

TTX keratinase is a specific alkaline serine protease extract from bacillus licheniformis by a liquid fermentation technique and refined by an advanced post-treatment technique.

It is a complex protease, including including reductase of disulfide bonds and polypeptide hydrolysis enzyme. Reductase of disulfide bonds acts on disulfide bond of keratin at first and break down macromolecular structure keratin into denatured protein, then denatured protein is degraded into polypeptide, oligopeptides and free amino acid by polypeptide hydrolysis enzyme, finally, keratin is disintegrated completely.

It is not only an alkaline protease, but also can break down disulfide bond, disintegrate keratin completely. So reductase of disulfide bonds in the keratinase is the key enzyme in the course of keratinolysis.

 

Advantage

1.A specific alkaline serine protease 2.Specialized in degrading keratin 3.Activity center includes serine 4.A compound protease including reductase of disulfide bonds and polypeptide hydrolysis enzyme

 

Specification

 

Name

Keratinase

Carrier

Starch

Activity

100,000 U/g, 200,000 U/g

Optimum pH

11(10.5-11.5)

Form

Powder

Valid pH range

6.0-12

Color

From white to kelly

Optimum temperature

60°C (50-60)

Loss on drying

≤8%

Valid temperature range

35-65°C

Packaging

20kg/bag

Storage

Six months under 25°C

Particle size

More than 80% pass through standard sieve of 40 meshes

 

Function of protease

1. Supplement the deficiency of endo-protease for animal.

2. Increase protein digestibility in animal feed and reduce the dosage of protein in feed formula.

3. Decrease the difference of feedstuff from different area and stabilize production performance.

4. Protect the environment and lessen pollution from nitrogen.

5. Develop out some new feedstuff e.g hydrolyzing feather meal, soybean meal and blood plasma powder.

 

Particular function of Keratinase

1. Degrade powerfully some anti-nutritional factors to be not broken down by trypsin and chymotrypsin e.g trypsin inhibitor, agglutinin etc.

2. Increase hydrolyzing degree of protein and proportion of soluble small protein in animal feed, which is conducive to animals’ absorption, e.g rise hydrolyzing degree 20% for corn protein, 10% for meat and bone meal, 5% for feather meal and soybean meal

3. Improve the digestibility of amino acid, especially for methionine, histidine and serine, keratinase have better action.

 

Definition of content

One unit (U) of keratinase activity is defined as the amount of enzyme which liberates 1 μg tyrosine per minute from substrate casein at PH 10.5 and 40°C. (GB/T 23527-2009)

 

Physical and chemical properties for keratinase

 

The application experiments on broiler

Divide 3000 AA broilers of 1 days into three group:

1.Control group: basal diet of corn soybean meal (CP 20.78%)

2.TestⅠ: low protein diet (CP 19.98%)

3.TestⅡ: low protein diet + keratinase 2000U/kg

The teat result as follow after 21 days:

1. Production performance of testⅠis lower than control group

2. production performance of TestⅡis higher tha control group, ADG increases 2.86%, feed conversion ratio reduces 0.07%

Above test result shows keratinase can reduce protein 0.8% and have no influence on production performance of broiler

 

 

Keratinase hydrolyzes feather meal

TTX keratinase hydrolyzes chicken feather for 6 hours at pH 10 and 55 degree centigrade, then test the content of amino acid by amino-acid andlyser and soluble protein with Bradford method.

 

The content of amino acid hydrolyzed out by TTX keratinase

Name

Content g/100g

Name

Content g/100g

NMDA

0.089

Tyrosine

0.49

Threonine

0.312

PHE

0.664

Serine

0.203

Lysine

0.228

Glutamic acid

0.361

Ammonia

0.021

Glycine

0.324

Histidine

0.106

Alanine

0.35

Arginine

0.052

Cystine

0.504

Proline

0.022

Valine

0.491

Tryptophan

/

Methionine

0.055

Total amino acid

4.842%

Isoleucine

0.173

 

 

Leucine

0.419

 

 

 

Keratinase is specialized in disulfide bond and degrade keratin, it can not only improve reaction efficiency but also increase conversion rate of feather meal, disintegrate completely feather meal.

 

 

 

 

The advantage of keratinase hydrolyzing feather meal

 

Method Total protein Soluble protein Soluble amino acid In vitro digestibility reaction condition

keratinase 82% 18.3% 4.84% 86% normal pressure and temperature

HTHP 81% 9.08% 2.03% 75% normal pressure and temperature

  Production process for keratinase

Packaging & Shipping

1. Packaging: Solid product: Paper-plastic composite bag, 25kg/bag or as requirement

                          Liquid product: 25kg/drum or 200 kg/drum or as requirement

2. Storage: Solid product: 12 months under 25℃ in cool and dry place.

                     Liquid product: 6 months under 25℃ in cool and dry place

Avoid the high temperature and direct sunlight.

After the package is opened, use early. The package should be sealed if it hasn’t been completely used.

3. Delivery date: 10-20 days after confirmed contract

4. Port of loading: Qingdao, China by sea or as requirement

One 20'GP can contain 10 -13 tons if bag packaging

1. Packaging: Paper-plastic composite bag, 25kg/bag or as requirement

2. Storage: 12 months under 25°C in cool and dry place. Avoid the high temperature and direct sunlight.

After the package is opened, use early. The package should be sealed if it hasn’t been completely used.

3. Delivery date: 10-20 days after confirmed contract

4. Port of loading: Qingdao, China by sea or as requirement

One 20'GP can contain 10 -13 tons if bag packaging

1. Packaging: 25kg/drum or 200 kg/drum or as requirement

2. Storage: 6 months under 25℃ in cool and dry place

Avoid the high temperature and direct sunlight.

After the package is opened, use early. The package should be sealed if it hasn’t been completely used.

3. Delivery date: 10-20 days after confirmed contract

4. Port of loading: Qingdao, China by sea or as requirement

One 20'GP can contain 10 tons if drum packaging

 

Why choose us?

1. We are manufacturer, we can produce high quality goods according to your requirement and OEM is available.

Founded in 1998, Jinan Tiantianxiang Co., Ltd. is specialized in feed additives. We can supply enzymes and feed additives for farmers, feed mill, traders and distributors in China and abroad. At present, we own two factories as production base. One specializes in flavor enchancement and other feed additives. The other specializes in enzymes used in animal feed, food and other industry..

We have built up production workshop 6500 square meters, including fermentation workshop, post-treatment workshop and spray drying workshop.The spray tower with evaporation 1.2 tons pressure is the largest spray tower in Chinese enzyme industry, height 36m, diameter 6.5m.

2. We have advanced independent R&D department.

Our R&d team include flavor chemists, animal nutritionist, and microbiologists. they are leading bio-feed additive manufacturer in China with the most advanced equipment.

The followed achievement were obtained in the last three years:

A. 14 products were commercialized

B. 3 Patent for Invention

C. 11 int’l patents have been allowed

D. 7 research projects received gov. award

3. We have advanced equipments, skilled workers and have established perfect system for quality control.

Product quality is the life of enterprise. How do we implement the quality control? Our company manage the quality control by the following ways:

At first, We prepared for a series of international quality system certifications and have acquired four cerificates: ISO9001, iSO 22000 (HACCP) , FAMI-QS and FDA

The second, Production and QC are all strictly performed under ISO and HACCP regulation.

The third, as mentioned before, we own R&d team including flavor chemists, animal nutritionist, and microbiologists and guarantee to update product technology and processing workmanship.

The fourth, after finishing researching, start to enter into production after pilot test.

At last, our QC team takes sample inspection on production field frequently in every process.

4. We have more than 19 years manufacturing experiences and more than 8 years exporting experience in this particular line.

5. Our professional services and experiences will make us be your trustful supplier.

 

 

Contact Us

We have been working hard.

We believe

We will become the most attractive supplier of feed additives and provides the highest value to the client as the provider enzymic preparation.

 

We are looking forward to your visiting us and becoming our cooperative partner.

  

 

Contact me
 Name Susan Lee 
 Company Jinan Tiantianxaing Co., Ltd.
 TEL +86-531-87159986
 FAX +86-531-87950220
 Skype Susan Lee_Jnttx
 Add 6F Quanjinghongyuan Business Building, 69 Yangguangxin Road, Jinan, China
 Website www.jnttx.com
Trade manager  

 

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